meet the team

Research Group Leader 

prof. RNDr. Daniel Růžek, Ph.D.


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Get to know more about Daniel!

During my high school years, I developed a profound passion for virology—a passion that remains unwavering to this day. After completing my undergraduate studies in Clinical Biology, I embarked on an academic journey that led me to obtain my Ph.D. from the University in South Bohemia and the Czech Academy of Sciences in the field of Molecular and Cellular Biology and Genetics, with a keen focus on Virology. Then I pursued postdoctoral training in Virology at the Texas Biomedical Research Institute in San Antonio, USA.
Following my enriching experience in the United States, I returned to my homeland with a renewed vigour and founded an independent research group dedicated to exploring various facets of virology. Central to our endeavours is an investigation into tick-borne encephalitis and other flaviviruses. My professional trajectory has been dedicated to unravelling the intricate interplay between viruses and their hosts, while also contributing to the development of countermeasures against these viral agents.
Beyond the confines of the laboratory, my interests encompass a diverse range of activities, such as travelling, hiking, cycling, and jogging. Furthermore, my fascination extends to mineral collection, and I am genuinely intrigued by architecture. Additionally, I am an aficionado of green tea and harbour a keen interest in all things related to Japan.

Senior Research Scientists

Get to know more about Luděk!

I studied at Masaryk University in Brno (molecular biology and genetics) and received my PhD from the same university (molecular and cell biology). My scientific career can be divided into three interrelated areas. As a PhD student under the supervision of Prof. Jiří Doškař, I studied the proteome structure of polyvalent staphylococcal bacteriophages, which could be used for phage therapy as an alternative to antibiotic treatment of severe bacterial infections. Later, I worked as a post-doctoral fellow at the Veterinary Research Institute in the research team of Dr. Milan Fránek. My research was focused on the design, development, optimization, and validation of antibody-based immunoanalytical assays (ELISAs) for quantitative determination of small molecules (contaminants, pollutants, and/or biomarkers) in environmental and food/feed samples. This part of my career was important for me because I got exposed to organic chemistry and started to love chemistry a lot. Since 2014 I have been working in the team of Prof. Daniel Růžek and my work is mainly focused on antiviral research, searching for new antiviral compounds/active agents, studying structure-activity relationships, explaining mechanisms of action of antiviral drugs and evaluating their antiviral activity in vitro and in animal models. In recent years I have also worked at several foreign workplaces (USA, Japan, Belgium, France). I am also a lecturer at several Czech universities and consider my teaching activities to be as important a part of my scientific career as research. In addition to biology (virology), I am interested in chemistry and physics, as there is no deep explanation of a biological process without a chemical and physical background. I am also very interested in art, especially drawing and painting, and feel a close connection between science, art and philosophy.

RNDr. Jiří Salát, Ph.D.

Head of the Laboratory of Emerging Viral Diseases at the Veterinary Research Institute


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Get to know more about Jiří!

I finished high school in my hometown Podbořany at the northwest of Bohemia. The first chapter of my scientific life is connected with České Budějovice where I started to study at the University of South Bohemia (Faculty of Sciences). I chose parasitology as a field of my master's and doctoral studies. At that time, I also worked in the Laboratory of Parasite Immunology at the Biology Centre, Czech Academy of Sciences (CAS). The leader of the laboratory was Jan Kopecký - an excellent mentor for the start of my scientific career. My research in that lab was focused mainly on the study of the immune response against microsporidians of the genus Encephalitozoon, which act as opportunistic pathogens in humans with immunodeficiency. Later on, I was engaged in the research topic “How ticks modulate the immune response of the host during infestation”. My research project was focused especially on tick saliva cystatins. During this period, I also spent two months at the Centre for Ecology and Hydrology (Oxford, UK) for training in recombinant protein production in the baculovirus expression system. I also collaborated with Daniel Růžek on studies of the immunopathology of tick-borne encephalitis (TBE) during these years. The second chapter of my scientific work is connected with Brno – metropolis of Moravia, where I started to work at the Veterinary Research Institute at the Department of Virology. My research area at this institution was focused firstly on the investigation of host-pathogen interactions of selected pig viruses (e.g. porcine reproductive and respiratory syndrome virus, porcine cytomegalovirus, African swine fever virus). Later on, I joined the group of Daniel Růžek - Emerging Viral Diseases. The mission of the laboratory is research in the area of epidemiology and pathogenesis of significant viral zoonoses, especially TBE. My projects in the team were focused mainly on the epidemiology studies of TBE presence in domestic animals (goats, sheep and dogs) and on TBE veterinary vaccine development. I was also involved in research connected with TBE therapy development (antivirals and therapeutic monoclonal antibodies) and immune response description. We also look for possible pathogens in nature (e.g. Brno virus) as a part of a collaborative project with the Institute of Vertebrate Biology, CAS. Nowadays, I am the head of the Laboratory of Emerging Viral Diseases at the Veterinary Research Institute, which continues the commenced studies. I also teach university students: the lecture “Parasite Immunology” at Masaryk University in Brno (Faculty of Science), and the practice lesson “Immunology” at Veterinary University in Brno. Besides science, I used to travel a lot to see and feel the world.

RNDr. Martin Palus, PhD

Laboratory of Arbovirology, Biology Centre of the Czech Academy of Sciences in České Budějovice


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Get to know more about Martin!

During my studies I became absolutely thrilled about the world of virology. My passion is unravelling the intricate mysteries of viruses – those tiny yet incredibly influential entities that shape the course of life. I believe that by understanding viruses, we can gain deep insights into the fundamentals of biology. If we want to stop the negative effects of viral infections, we should first understand disease development. So, after finishing my studies in Clinical Biology, I started my PhD in Infectious Biology within the joint School of Doctoral Studies in Biological Sciences between the Faculty of Science, University of South Bohemia, and the Biology Centre of the Czech Academy of Sciences. I gained experience through my work in the Laboratory of Biological Monitoring and Protection, The National Institute for Nuclear, Chemical and Biological Protection, internships such as: The Roslin Institute and Royal (Dick)School of Veterinary Studies, The University of Edinburgh; Immunology and Infectious Disease, Institute for Research in Biomedicine, Switzerland etc. Currently, I am working as head of the Laboratory of Arbovirology, Institute of Parasitology, Biology Centre CAS. My main interest is flaviviruses and other emerging and re-emerging viral infections. Besides research, I enjoy spending time with my family and engaging in activities such as fishing, parrot breeding, and home improvement.

Mgr. Václav Hönig, PhD

Laboratory of Arbovirology, Biology Centre of the Czech Academy of Sciences in České Budějovice


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Get to know more about Václav!

After completing my master's degree in microbiology at the Faculty of Science Masaryk University, I started my PhD in parasitology within the joint School of Doctoral Studies in Biological Sciences between the Faculty of Science, University of South Bohemia, and the Biology Centre of the Czech Academy of Sciences. During my PhD, I also worked as the head of the laboratory and research and development department of the private company Gen-Trend. Currently, I work in the Laboratory of Arbovirology, Institute of Parasitology, Biology Centre CAS. I am interested in the ecoepidemiology of zoonotic and vector-borne pathogens (tick-borne encephalitis virus, Usutu virus, hantaviruses, and other tick-, mosquito-, and rodent-borne pathogens including bacteria) and the ecology of their vectors (ticks, mosquitoes). In addition to research, I enjoy spending time with my family, ideally doing outdoor sports (trail biking, trail running, snowboarding, skiing, hiking, climbing etc.).

Research Associate

Mgr. Andrea Fořtová, Ph.D.


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Get to know more about Andrea!

After graduating from Masaryk University in Brno (Faculty of Science), I worked for 15 years in Loschmidt Laboratories (MU) on protein development of haloalkane halogenase and for 7 years in RNA quality control group (CEITEC). In 2020, I entered the world of viruses in Daniel Ruzek's lab, which still fascinates me. In my free time I enjoy the mountains in summer and winter, love Stephen King‘s books and gardening.

Postdocs / Junior Research Scientists

Alexandra Wilson, Ph.D.


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Get to know more about Alex!

My passion for science started when I was quite young, and although I always had a particular interest in virology, I wanted to develop a broader skill set before I specialised. This led me to completing an integrated masters in molecular and cellular biochemistry (MBiochem) at the University of Oxford. From there, my academic journey took me to the university of Glasgows MRC - Centre for Virus Research in Scotland for my PhD. The focus of my work was tick-borne bunyaviruses, more specifically trying to understand why these viruses were only able to be transmitted by ticks (as opposed to being able to be vectored by other arthropods, such as mosquitoes). Through this research, I developed a love for all things tick-virus related, which is what has led me to Brno and the lab of Prof. Daniel Růžek, where I am exploring the mechanisms behind alimentary routes of infection. I’m very excited to research all things tick-borne encephalitis virus related, continue improving my skill set, and explore a new country as this is my first time living outside the UK. Outside of the lab, I love to do anything craft related (particularly cross stitching), spend as much time in nature and hiking as possible, and consume anything horror genre related (mainly books and films).

Mgr. Jiří Holoubek, Ph.D.


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Get to know more about Jiří!

I graduated from Microbiology and Molecular Biotechnology at Masaryk University in Brno. My beginnings in the lab were influenced by bacteriophages, which led me to virology. Now I am totally fascinated by flaviviruses (both tick- and mosquito-borne and the differences between them). Although I love many aspects of virology (including tons of plaque assays), my current focus is primarily on molecular pathogenesis, virus-host interactions, and antiviral drug development. I have also become a fan of fluorescence microscopy, so I am trying to expand my knowledge and skills in bioimaging (confocal, OPT, LSFM) and its application in various virology projects. Outside of the lab, I enjoy hiking in the mountains and traveling around the world. And I love coffee and chocolate!

PhD students

Mgr. Michaela Beránková


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Get to know more about Michaela!

I am a second-year PhD student, a molecular biologist by education and heart, fascinated by the fusion of molecular biology and virology. The world of viruses truly amazes me - it's mysterious and full of surprises. Currently, my focus in the lab revolves around viral reporter systems, where I'm untangling their secrets. When I'm not busy pipetting, you can catch me experimenting with flavors in the kitchen or embarking on hikes somewhere up high. The kitchen and lab are my creative spaces, and hiking is my way of finding new perspectives.

Mgr. Petr Bednář


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Get to know more about Petr!

After graduating from Masaryk University I spent several years in the pharmaceutical industry, where I was focused on veterinary virology and vaccine production. Then I moved back to academia and started my PhD studies at the University of South Bohemia. Now I focus on tick-borne encephalitis virus and the preparation of its mutants. I am also "that guy with a toolbox" in our lab. I love good coffee, good meal, good wine and good book and it doesn´t have to be all at a time.

Mgr. Martin Macháček

Get to know more about Martin!

I guess my first spoken word being „bird“ somehow predicted my interest in biology (or maybe it was the first manifestation of it). My fondness for biology resulted in me studying molecular biology and genetics at Masaryk University. During my studies I started to work on microalgae, to be more precise I was attempting to increase the production of lipids in Schizochytrium, which is utilized in the industrial production of fish oil alternatives. After graduation, I have decided to switch from big microorganisms to small microorganisms and joined this virology lab. Besides biology, I also like Irish music and the history of the USA. And when I am not explaining how these three things have undeniable historical connections I enjoy playing some board games.

Mgr. Tereza Frčková

Get to know more about Tereza!

I have been fascinated by science and nature since childhood, mainly the invisible part of nature. I went to study Molecular biology and the biochemistry of organisms at Charles University in order to better understand the things around us. When I was choosing the lab, I was deciding between virology and cancer research. So I ended up in the lab of tumor virology, where my topic was the integration of the virus genome into the human chromosome and its association with tumorgenesis. Besides my research work, I try to pass on science enthusiasm to the younger generation. Now I am looking forward to learning new methods and exploring other viruses.

Mgr. Zuzana Krátka

Get to know more about Zuzana!

My life has always been guided by two fundamental passions: learning and creating. After earning a bachelor's degree in Molecular Biology and Genetics from Masaryk University, I was fortunate to begin pursuing my first passion in Professor Růžek's lab, where I focused on novel approaches to flaviviral infection therapy, particularly through drug repurposing, which ultimately led to earning my master's degree. For my PhD, I chose to continue exploring the world of flaviviruses, now delving into the molecular, immunological, and genetic factors that contribute to severe cases of tick-borne encephalitis. Beyond science, I find joy in painting, poetry, knitting, crocheting, and beading jewelry. I am continually amazed by how these seemingly unrelated interests—science and art—often intersect and inspire each other, offering new perspectives.

MUDr. Michal František Kříha

extramural study

Get to know more about Michal!

After completing my medical studies at Charles University in Prague, I started working at the Infectious Diseases Department, České Budějovice Hospital. In addition to the routine clinical practice, I had the opportunity to join the ongoing research collaboration with the Biological Center of the Academy of Sciences. This collaboration led to my doctoral study under Prof. Růžek's supervision at the Faculty of Science, University of South Bohemia. It includes inventing new methods for the clinical diagnosis and treatment of tick-borne encephalitis. In my free time I enjoy playing board games, organizing tea sessions, trail running and choir singing.

Technicians and Lab Managers

Mgr. et Mgr. Michal Dvořák

Lab Project Manager & Lab Specialist


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Get to know more about Michal!

During my studies, I developed interest for pathogenic microorganisms and infectious diseases. For that reason, I studied Microbiology and Immunology at Masaryk University in Brno. Firstly, I focused on tick-borne microorganisms, such as Borrelia burgdorferi s.l., Anaplasma phagocytophilum, Francisella tularensis, Rickettsia spp. and Coxiella spp. After that, I bacame more interested in virology and I studied tick-borne encephalitis, especially in relation to obesity and type II diabetes. Throughout my career, I also performed laboratory diagnostics of the SARS-CoV-2. Although I am fascinated by many aspects of virology, I am currently most interested in the tick-borne encephalitis virus and other flaviviruses. I am interested in pathogenesis, interaction of pathogens with the host and their immune system. I am experienced in methods of molecular biology, immunology and virology (PCR, plaque assays, ELISA tests, VNT, etc.). Currently, I am working in the laboratory of Virology as a Project Lab Manager and Lab Specialist. I deal with administrative agenda related to the projects and I also perform routine and specialized laboratory methods within the laboratory of Virology. Outside the laboratory, I engage in a wide range of activities such as travelling, hiking, running, cycling, etc. I like nature, which constantly fascinates me. I am a passionate lover of tea and tea culture. In my free time I also play various board games and I also like reading books, especially detective and fantasy.

Mgr. Karel Vostal

Administrative Assistant


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Get to know more about Karel!

I graduated from Animal physiology at the Faculty of Science in Brno. For several years I worked there as a researcher. After a break from science, I returned as an administrative assistant for Prof. Růžek's group. Besides biology, I am interested in history, music and films. I like to go on long hikes, play billiards and board games.

Undergraduate students

Bc. Eliška Mráčková


Bc. Yukako Tamazawa


Bc. Amir Mugutdinov


Mgr. Markéta Doležalová


Bc. Tomáš Kořánek


Bc. Sára Lukášová


Bc. Jana Bednářová


Alumni

Postdocs

Soumajit Mukherjee – now King's College London, UK

Lucie Dufková – now Czech Centre for Phenogenomics, Prague

Petra Straková – now Veterinary Research Institute, Brno

Ganesh Duraisamy – now Mendel University, Brno

 

PhD

Pavel Svoboda (2023) – now Institute of Organic Chemistry and Biochemistry, Prague

Jan Haviernik (2020) – now University of Otago, New Zealand

Jana Elsterová (2020) – now on maternity leave

Petra Formanová (2018) – now self-employed

Jiří Černý (2016) – now Czech University of AgriSciences, Prague

Martin Palus (2016) – now Biology Centre of the Czech Academy of Sciences, Ceske Budejovice

 

MSc.

Daša Ochodničanová

Michal Dvořák 

Michal Štefánik

Ondřej Pěnčík

Hana Al Kirbiová

Jana Koutná

Marie Uhlířová

https://mik.sci.muni.cz/ruzeklab

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